Abstract: The production of lipids in this paper was studied in the fungus Mortierella isabellina As3.2793. Two growth variables were systematically manipulated. The effect of various carbon and nitrogen sources and their concentritions on mycelium growth and accumulation of lipid yield in shaker flask culture was studied. We came out conclusion that glucose derived from maize starch hydrolysate was the sole carbon source in culture medium at concentration of 100g/L, and yeast extract paste was most effective for fermentation consisting of 3g/L. The dry biomass under optimum conditions was 12.350g/L, and consisted of 52.19% lipid. The lipid per liter culture was 6.4376g. It is remained to optimize other ingredients in culture medium to enhance lipidy ield.

Key words: lipid, Mortierella isabellina, carbon source, nitrogen source