Screening of Fusants Derived from Protoplast Fusion of Bifidobacterium with Saccharomyces cerevisiae

Abstract

Abstract: Objective: To study on the screening of fusants derived from protoplast fusion of Bifidobacterium with Saccharomyces cerevisiae. Method: The fusants were derived from protoplast fusion between Bifitdobacterium bifidum ATCC29521 and Saccharomyces cerevisiae CICC1856. 10% lactose culture medium was adopted in first screening. The stability of further generations and biology characteristics of the fusants were analyzed. DNA of the fusants and their parents were investigated with fluorescency in situ and hybridization probed with bifidobacterium oligonucleotide. Result: The fusants possessed had the special DNA sequence of Bifidobacterium and biological characters of both Bifidobacterium and Saccharomyces cerevisiae. Conclusion: Fusants derived from Bifidobacterium and Saccharomyces cerevisiae protoplast fusion are examples of making anaerobe and yeast intra-kingdom fusion realize. Its success undoubtedly offers new ways in studying of bifidobacterium species’ improvement as well as in development and utilization of its biological function in the future .

Key words: Bifidobacterium, Saccharomyces cerevisiae, protoplast fusion, fusant

Li-Yong-Xue, ZHANG De-Chun, LI Dai-Kun. Screening of Fusants Derived from Protoplast Fusion of Bifidobacterium with Saccharomyces cerevisiae[J]. FOOD SCIENCE, 2006, 27(2): 84-86 https://doi.org/

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Screening of Fusants Derived from Protoplast Fusion of Bifidobacterium with Saccharomyces cerevisiae

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