Abstract: The method of extracting and purifying polysaccharide from Momordica charantia was suggested in this paper. Itsphysical and chemical characteristics and biological activity were determined. The polysaccharide was extracted from Momordicacharantia by adding two species of enzymes. The optimum hydrolysis conditions were got. The polysaccharide was furtherpurified with DEAE-32 column chromatography and Sephadex G-75 column chromatography respectively until the single peakwas got, and named as PMCⅠ. The acidic hydrolysis solution of PMCⅠwas analysed by means of TLC: its constituentsrespectively glucose, galactose, mannose, rhamnose. The study for its biological activity showed that it could eliminate free radicalssuch as ·OH and O2·, and its IC50 for ·OH and O2· were respectively 0.0017mg/ml and 0.005mg/ml. So it showed theactivity of antioxidant. The experiments of inhibiting germs show that PMCⅠcan inhibit germs such as S.Aureus, E.coli, B.subtilusand S.typhimurium. The IC50 for those were respectively 0.10, 0.15, 0.13, and 0.13mg/ml, the most remarkable of which was S.Aureus.

Key words: Momordica charantia polysaccharide, seperation purification, cellulase, activity- -