Determination of Saccharides Components in Orange Juice by HPLC

Abstract

Abstract: Determination of saccharides in orange juice was studied by high performance liquid chromatography. Saccharides were extracted from samples with water by oscillation and isolated by solid phase extraction with Sep-Pak-C18 cartridge. Sucrose, glucose and fructose were separated on an Agilent Zorbax Carbohydrate column(4.6×250mm, 5μm), with 80% acetonitrile solution as mobile phase, around temperature 25±2℃. The relative standard deviations are 0.83%～1.71% and the recoveries are 96%～103%. This method can be applied to the determine saccharides components in orange juice.

Key words: High Performance Liquid Chromatography(HPLC), saccharide, orange juice, solid phase extraction

LI Yun-Kang, PAN Si-Yi. Determination of Saccharides Components in Orange Juice by HPLC[J]. FOOD SCIENCE, 2006, 27(4): 190-192.

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Determination of Saccharides Components in Orange Juice by HPLC

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