FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (11): 81-85.

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Isolation and Identification of Saccharides from Hohenbuehelia serotina and Its Effect on Scavening Oxygen Free Radicals

 YANG  Li-Hong, LIU  Lin-De, JIANG  Hua, WANG  Xiao-Jie, XING  Wei, CAI  De-Hua   

  1. 1.Department of Biological Science and Technology, Ludong University, Yantai 264025, China; 2.Department of Chemistry and Materials, Ludong University, Yantai 264025, China
  • Online:2007-11-15 Published:2011-11-22

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Abstract: The saccharides of Hohenbuehelia serotina were extracted by hot water and then was deposited by ethanol and the DEAE cellulose column chromatography was used on the polysaccharide purification after the protein was excluded with Sevag method. The result indicated that the polysaccharide was composed of HsI and HsII. The Sepharose CL-4B method showed that molecular weights of HsI and HsII were all 2.7725×104 when Infrared Ray(IR) and Magnetic Resonance(NMR)assays showed that HsI and HsII were all D-glucopyranose (containing two kinds of configuration,αandβ, which αtoβof HsI was 1:1 while αtoβof HsII was 2:1), and they were all linked by 1-4 linkage and 1-6 linkage which the ratio of HsI was 1:1 and the ratio of HsII was 2:1. The pyrogallic acid autooxidation method, according to its assays on scavening oxygen free radicals, indicated that both polysaccharides and the pure HsI and HsII have anti-oxidant properties.

Key words: Hohenbuehelia serotina, polysaccharide, isolation, oxygen-derived free radicals