Abstract: Three strains(NN22, SS22, QS29) of di-mon mating and monokaryon strain S2 of Lentinula edodes were iron-enriched in the medium with Fe2+ content ranged from 0.01 to 0.04 mg/ml. The optimization of cultivation conditions of NN22 strain, which is highest yield of iron-enriched one was selected. The results showed that the highest Fe2+ content of NN22 strain was obtained within 20 days at 25℃ and initial pH7.0 in 0.02 mg/ml Fe2+ of PDY. Under the optimized cultivation conditions, Fe2+ content and polysaccharide content of NN22 strain reached 348μg/g and 5.18 mg/g respectively. The results also showed that esterase isoenzyme of NN22 strain was activated when the concentration of iron was less than 0.02 mg/ml.

Key words: Lentinula edodes, iron-enriched, cultivation condition, polysaccharides, esterase isozyme