Abstract: Objective: Accelerated solvent extraction (ASE) was applied to extract six sulfonamide residues in animal tissues, and compared with ultrasonic-assistant extraction or homogenization extraction. Methods: Various extracting parameters were investigated to optimize the extracting conditions that would affect the recovery of sulfonamides during ASE. Results: The suitable conditions of ASE are as follows: samples extracted with dichloromethane, operating pressure is 500 psi, temperature 40℃, extracting time 23 min and the collected volume 12 ml. Recovery of any sulfonamides is higher than 80% and the relative standard deviation (RSD) less than 9%. Conclusion: Comparing with traditional method, the ASE is rapid and effective.

Key words: accelerated solvent extraction, sulfonamides, animal tissue