FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (2): 158-162.

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Study on Arachidonic Acid Mutation Strains Breeding of Diasporangium sp.

WANG  An-Qi, DAI  Chuan-Chao, ZHAO  Mo, ZHAO  Qing-Xin, ZHOU  Lei-Lei   

  1. 1.Key Laboratory of Microbial Engineering, College of Life Science, Nanjing Normal University, Nanjing 210097, China; 2.College of Life Science and Biotechnology, Yancheng Teachers College, Yangcheng 224002, China
  • Online:2007-02-15 Published:2011-12-31

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Abstract: Using Diasporangium sp. as original strain, with low temperature 15℃ and 0.09 mg/ml aspirin as inhibitors, after UV radiation, some mutation strains were selected. Fermentation experiments assayed various strains in the biomass, arachidonicacid yield and fatty acids. The changes of fatty acids showed that the aspirin is the most effective inhibitor, and A1.13 strain is selected with its arachidonic acid yield enhanced 53.15%.

Key words: arachidonic acid, Diasporangium sp., mutation breeding