Study on Conditions of Ganoderma Protoplasts Fusion

FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (2): 173-176.

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Study on Conditions of Ganoderma Protoplasts Fusion

CHEN He, HU Yun-Hong, QIN Jun-Zhe, GU Shen-Rui

1.College of Life Science and Engineering, Shaanxi University of Science and Technology, Xianyang 712081, China; 2.Enzyme Engineering Institute, Shaanxi Province Sciences Academy, Xi’an 710600, China

Online:2007-02-15 Published:2011-12-31

Abstract

Abstract: The protoplast fusion conditions between Ganoderma lucidum(GL) protoplast and Ganoderma applanatum(GA) protoplast were compared with chemical fusion and electrofusion. the optimum conditions of protoplast in chemical fusion are: 30% PEG (polyethylene glycol), 0.02mol/L CaCl2 and 30℃ for 30min, with the fusion rate 0.4% , whereas the optimum conditions of protoplast in electrofusion are: voltage 40V, frenquency 2MHz, direct pulse voltage 500V, vector 10kV/cm, pulse 25μs, with much higher fusion rate 40%.

Key words: Ganoderma, protoplast, electro-fusion, chemical fusion, breeding

CHEN He, HU Yun-Hong, QIN Jun-Zhe, GU Shen-Rui. Study on Conditions of Ganoderma Protoplasts Fusion[J]. FOOD SCIENCE, 2007, 28(2): 173-176.

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Study on Conditions of Ganoderma Protoplasts Fusion

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