Synthesis and Identification of the Hapten N-(3,5-Dichlorophenyl) Succinimide-2-Hemisuccinate for Immunoassay

FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (2): 223-226.

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Synthesis and Identification of the Hapten N-(3,5-Dichlorophenyl) Succinimide-2-Hemisuccinate for Immunoassay

LIU Rong-Rong, LIU Xian-Jin, LIU Yuan, FANG Dun-Huang, HU Qiu-Hui

1.College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2.Pesticide Evaluation Laboratory, Jiangsu Academy of Agriculture Science, Nanjing 210014, China; 3.Yunnan Tobacco Research Institute, Yuxi 653100, China

Online:2007-02-15 Published:2011-12-31

Abstract

Abstract: Dimethachlon is a kind of small molecular hapten that has immunoreactivity instead of immunogenicity. In order to establish an immunoassay method for dimethachlon, N-(3,5-dichlorophenyl)-2-hydroxysuccinimide was synthesized by chloralide of malic acid and 3,5-dichloroaniline, and then reacted with succinic anhydride to obtain the hapten N-(3,5- dichlorophenyl) succinimide-2-hemisuccinate. The structure of the product was identified by IR, 1HMR and LC-MS, which provided the foundation for future research of dimethachlon immunoassay.

Key words: dimethacholn, hapten, syntensize

LIU Rong-Rong, LIU Xian-Jin, LIU Yuan, FANG Dun-Huang, HU Qiu-Hui. Synthesis and Identification of the Hapten N-(3,5-Dichlorophenyl) Succinimide-2-Hemisuccinate for Immunoassay[J]. FOOD SCIENCE, 2007, 28(2): 223-226.

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Synthesis and Identification of the Hapten N-(3,5-Dichlorophenyl) Succinimide-2-Hemisuccinate for Immunoassay

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