FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (6): 201-204.

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Expression of Oral Hepatitis B Vaccine in Transgenic Tomato

HAO  Hao-Yong, WEI  Ya-Hui, ZHU  Jian-Guang, SUN  Jie, WANG  Ya-Ning, JING  二Yong, ZHANG  Bian-Ling, XUE  Ke   

  1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, China
  • Online:2007-06-15 Published:2011-12-31

Abstract: Constructed plant expressive vector pCAMBIA1301/HB was changed into Agrobacterium tumefaciens LBA4404 by which tomato was transformed. By PCR and Southern blotting, regenerated plants were validated that HBsAg gene is transformed successfully. Notable distinction of protein between transformed and untransformed plants was observed by SDS- PAGE. ELISA and Western blotting indicated HBsAg has expressed in transformed plants,with MW about 24 kD.

Key words: oral vaccine, HBsAg, tomato, transgenic plant