Abstract: The effect of carbon sources, nitrogen sources and different concentration of inorganic salt on the production of exopolysaccharide of Pholiota adiposa was studied in this paper by liquid culturing and orthogonal test. The results showed that the suitable carbon sources for exopolysaccharide produced by Pholiota adiposa were sucrose, maltose and brown sugar and the suitable nitrogen sources were wheat bran, yeast extract power and NH4NO3. The best liquid medium proved by the orthogonal experiment was sucrose 2%, wheat bran 3%, MgSO4 0.15% and KH2PO4 0.25%.

Key words: Pholiota adipose, exopolysaccharide, orthogonal test, carbon sources, nitrogen sources