HPLC Determination of Trigonelline in Pumpkin Powder

Abstract

Abstract: objective: To set up a method for determination trigonelline in pumpkin powder by HPLC. Method: Pumpkin powder sample was extracted with methanol ultrasonic, Zorbax-NH2(4.6×150 mm, 5μm) column was used with mobile phase as acetonitrile-water (80:20,V:V). The flow rate was 1ml/min and detection wavelength was set at UV 265 nm. Result: The calibration curve is linear in the range of 5.01～101.4μg/ml, with the correlation coefficient 0.9999(n=5); the RSD 0.31(n=5) in the precision test, the average recovery rate 85.26% and RSD 4.4%(n=6). The contents of trigonelline in the three kinds of thirty samples are 0.1219～0.4836 mg/g. Conclusion: The method can provide scientific indexes for developing the functional compo-nent and selecting breed of pumpkin.

Key words: pumpkin powder, trigonelline, HPLC

ZHANG Ying-Chun, YANG Xin, ZHANG Hua, WANG Jing, XU De-Chang. HPLC Determination of Trigonelline in Pumpkin Powder[J]. FOOD SCIENCE, 2008, 29(1): 280-282.

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HPLC Determination of Trigonelline in Pumpkin Powder

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