Development of MPCR-DHPLC Detection Method for Five Pathogenic Vibrios in Foods

Abstract

Abstract: The genome DNAs of five pathogenic Vibrios were extracted by kit method,including Vibrio cholerae,V. parahaemolyticus,V. vulnificus,V. alginolyticus and V. mimicus,and their five pairs of special primers were composed,respectively. The MPCR-DHPLC detection methods among the five vibrios were developed after the PCR reaction systems were optimized. The detection sensitivity of duplex PCR-DHPLC is 100 CFU/ml. The developed MPCR-DHPLC method has good application merit on detecting the Vibrios in foods.

Key words: Vibrio, genome DNA, multiplex PCR (MPCR), denaturalization high performance liquid chromatography (DHPLC)

SHAO Bi-Ying, WANG Chuan-De, ZHENG Jing, HUANG Xiao-Rong, CAO Ji-Juan, CHEN Bin, WU Qian. Development of MPCR-DHPLC Detection Method for Five Pathogenic Vibrios in Foods[J]. FOOD SCIENCE, 2008, 29(10): 500-504.

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Development of MPCR-DHPLC Detection Method for Five Pathogenic Vibrios in Foods

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