Study on Screening of Broad Spectrum Lactolin-producing Lactic Acid Bacteria and Its Separation and Purification from Fermentation Broth

Abstract

Abstract: The aim of this experiment was to screen broad spectrum lactolin-producing strains,extract and purify the lactolin from fermentation broth.Twenty-two lactolin-producing lactic acid bacteria were screened out from several material,and the strain B9,the lactolin activity produced by which is the highest,was selected through Oxford cup method.This strain was identified as Lactobacillus plantarum by physiological and biochemical experiments.The diameter of inhibition zone produced by the broth of the strain B9 is 22.19 mm.The broth was purified with the process of salting-out of(NH4)2SO4,dialysis desalting,DEAE-52 column chromatography and Sephadex G-25 column chromatography.The diameters of inhibition zone of the products in all the procedures are 19.54,23.46,25.32 and 25.76 mm,respectively.The final product was detected to be one peak with high performance liquid chromatography(HPLC).The purity of the lactolin produced by this strain is increased greatly throgh the series of experiments.The lactolin produced by this strain has strong activity against not only gram-negative bacteria like Escherichia coli,but also gram-positive bacteria like Staphylococcus aureus.Therefore,it has a wide spectrum against bacteria.

Key words: lactolin, screening, identification, purification

MOU Guang-Qing, LI Xia, LI Hui, HUO Gui-Cheng. Study on Screening of Broad Spectrum Lactolin-producing Lactic Acid Bacteria and Its Separation and Purification from Fermentation Broth[J]. FOOD SCIENCE, 2008, 29(11): 469-472.

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Study on Screening of Broad Spectrum Lactolin-producing Lactic Acid Bacteria and Its Separation and Purification from Fermentation Broth

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