FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (8): 496-498.

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Analysis of Seven Kinds of Pyphenol Compounds in Propolis Extracts by High Performance Liquid Chromatography

 SUO  Zhi-Rong, CAO  Wei, QIN  Hai-Yan   

  1. 1.Southwest University of Science and Technology,Mianyang 621010,China;2.Northwest University,Xi’an 710069,China
  • Online:2008-08-15 Published:2011-08-26

Abstract: Objective:To establish a HPLC method for determining of seven kinds of polyphenol compounds in ninetypes of propolis extracts simultaneously. Method:RP-HPLC was used. The polyphenol compounds was analyzed on Zorbax SB-C18 column(150 mm×4.6 mm,5.0μm) and by gradient elution program performed with methanol and 1% formyl acid aqueous as a mobile phase. The flow rate was 1.0 ml/min. The detection was done at 280 nm and column temperature was 30℃. Results:Seven kinds of polyphenol compounds are separated satisfactorily with good linear relationship. Conclusion:A rapid,sensitive and accurate method for the determination of seven kinds of polyphenol compounds is founded. This study can provide a scientific basis for the quality control of propolis.

Key words: high performance liquid chromatography, propolis extracts, polyphenol compounds