Double-enzyme Method for Polysaccharides Extraction from Gomphidius rutilus Fruitbodies

doi:10.7506/spkx1002-6630-201018033

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (18): 143-146.doi: 10.7506/spkx1002-6630-201018033

• Processing Technology • Previous Articles Next Articles

Double-enzyme Method for Polysaccharides Extraction from Gomphidius rutilus Fruitbodies

LI Shu-qian1,2，ZHANG Bo1，XIN Guang1，YU Yang1，LIU Chang-jiang2,*

1. Department of Chemistry, Anshan Normal University, Anshan 114007, China；2. College of Food, Shenyang Agricultural University, Shenyang 110161, China

Received:2010-06-07 Revised:2010-07-27 Online:2010-09-25 Published:2010-12-29
Contact: LIU Chang-jiang2,* E-mail:liucj597@sohu.com

Abstract

Abstract:

For polysaccharide extraction, wild Gomphidius rutilus fruit bodies were hydrolyzed with cellulase and papain together. Based on single factor investigations, orthogonal array design was employed to optimize the hydrolysis process, and the optimum hydrolysis conditions were found as follows: hydrolysis temperature 60 ℃; cellulase/papain mass ratio 3:1; pH 5.0; and hydrolysis duration 3 h and the polysaccharide yield obtained under such conditions was 12.0%. This method has many advantages over conventional methods, such as gentle reaction conditions, time saving and less destructive effect on polysaccharide structure.

Key words: Gomphidius rutilus, polysaccharide, extraction, compound enzymes

CLC Number:

TS201.22

LI Shu-qian1,2，ZHANG Bo1，XIN Guang1，YU Yang1，LIU Chang-jiang2,*. Double-enzyme Method for Polysaccharides Extraction from Gomphidius rutilus Fruitbodies[J]. FOOD SCIENCE, 2010, 31(18): 143-146.

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Double-enzyme Method for Polysaccharides Extraction from Gomphidius rutilus Fruitbodies

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