Rapid Detection of Enterobacter sakazakii by LAMP

doi:10.7506/spkx1002-6630-201022070

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 322-325.doi: 10.7506/spkx1002-6630-201022070

• Analysis & Detection • Previous Articles Next Articles

Rapid Detection of Enterobacter sakazakii by LAMP

MA Yin-zhong1，CHEN Jiang-yuan2，FANG Guo-liang1，LI Qi1，LI Rui1，ZHOU Guo-ping1，LIU Zhi-guo1,*

(1. School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China；
2. School of Health Science, Jianghan University, Wuhan 430016, China)

Received:2010-06-30 Revised:2010-10-11 Online:2010-11-25 Published:2010-12-29
Contact: LIU Zhi-guo1 E-mail:zhiguo_l@126.com

Abstract

Abstract:

Objective: To develop a loop-mediated isothermal amplification (LAMP) method for rapid detection of Enterobacter sakazakii in food. Methods: LAMP primers were designed on the basis of 16S rRNA gene (16S rDNA) and outer membrane protein A gene (OmpA) in Enterobacter sakazakii. The LAMP products were evaluated by electrophoresis or SYBR-GREEN I. Results: The detection limit of Enterobacter sakazakii by LAMP assay was 32 CFU/mL using 16S rRNA primers or 3 CFU/mL using OmpA primers. The similar detection sensitivity of LAMP products using SYBR-GREEN I and electrophoresis was observed. Conclusion: LAMP method was effective and sensitive for rapidly detecting Enterobacter sakazakii, which will have a broad application prospect in food.

Key words: Enterobacter sakazakii, loop-mediated isothermal amplification, detection

CLC Number:

Q93.3

MA Yin-zhong1，CHEN Jiang-yuan2，FANG Guo-liang1，LI Qi1，LI Rui1，ZHOU Guo-ping1，LIU Zhi-guo1,*. Rapid Detection of Enterobacter sakazakii by LAMP[J]. FOOD SCIENCE, 2010, 31(22): 322-325.

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Rapid Detection of Enterobacter sakazakii by LAMP

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