Protective Effect and Mechanism of Apostichopus japonicus on Kidney of Diabetic Rats

Abstract

Abstract: Objective: To explore the protective effect of Apostichopus japonicus on the kidney of streptozotocin (STZ)-induced diabetic rats and its possible mechanisms. Methods: A diabetic rat model was developed by a single intraperitoneal injection of STZ at the dose of 60 mg/kg and kidney damage was induced by long-term high levels of blood glucose. The diabetic rats were administered with Apostichopus japonicus at the daily dose of 800 mg/kg for 8 consecutive weeks. The changes in fasting blood glucose, urine glucose, renal index, and the excretion amounts of urine protein, mAlb and NAG were determined. The mRNA expression of peroxisome proliferation activated receptor-γ (PPARγ), transforming growth factor beta 1 (TGF-β1) and connective tissue growth factor (CTGF) in kidney were assessed by RT-PCR. Results: Apostichopus japonicus showed remarkable a suppressive effect on fasting blood glucose, uric glucose, kidney index and the excretion amounts of protein, mAlb and NAG in diabetic rats. The expression level of PPARγ mRNA was markedly increased, while the mRNA expression levels of TGF-β1 and CTGF were effectively suppressed by Apostichopus japonicus. Conclusion: Apostichopus japonicus can remarkably reduce blood glucose through improving the expression of PPARγ and restraining the expression of TGF-β1 and CTGF, which results in a protective effect on kidney damage.

Key words: diabetes, Apostichopus japonicus, PPARγ, TGF-β1, CTGF

CLC Number:

References

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