Effect of Lactobacillus acidophilus on β-Lactoglobulin Induced Mouse Th1/Th2 Cell Imbalance

Abstract

Abstract: Objective: To investigate the effects of Lactobacillus acidophilus on the Th1/Th2 cell balance and antibody production in bovine β-lactoglobulin (BLG)-sensitized mice and evaluate its efficacy to relieve consequent allergic reactions. Methods: BALB/c mice were intraperitoneally injected with a mixture of milk BLG and Freund,s adjuvant to create BLG-sensitized mouse models. The mice were randomly divided into blank group, allergic group and different dose of Lactobacillus acidophilus group. The total IgE, BLG-specific IgE and total IgG contents in the serum of mice were measured by ELISA. Splenic lymphocytes were isolated and cultured in vitro to detect the levels of Thl type cytokine (IL-12, IFN-γ) and Th2 type cytokine (IL-4) in the supernatant by ELISA. Results: The IFN-γ/IL-4 ratio, which represents the Th1/Th2 cell balance, increased significantly in both middle- and high-dose Lactobacillus acidophilus groups compared to the allergic group (P＜0.05). At the same time, their serum levels of total BLG-IgE and total IgG decreased significantly (P＜0.05), but showed no significant difference compared to the blank control (P＞0.05). Conclusion: Intragastric administration of Lactobacillus acidophilus may be effective in preventing and alleviating BLG allergic symptoms by restoring the Th1/Th2 cell balance to Th1 dominance and by inhibiting IgE and IgG production.

Key words: Lactobacillus acidophilus, β-lactoglobulin, milk allergy

CLC Number:

R392

References

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