Secondary Structure of Bifunctional Chitosanase-Cellulase from Trichoderma viride as Determined by FT-IR Spectrometry

Abstract

Abstract: The secondary structure of two bifunctional chitosanase-cellulase enzymes (CCBEⅠ and CCBEⅡ) from Trichoderma viride was explored by FT-IR spectroscopy. Amide Ⅰ and Ⅲ FT-IR bands allowed prediction of the secondary structure of CCBE. The errors of determination for α-helix and β-sheet contents in CCBEs were less than 2% and 4%, respectively. The quantitative analysis of CCBEⅠ revealed that the amino acid sequence was consistent with the results of circular dichroism analysis and Garnier-Robson prediction. The β-sheet content was approximately 70% in CCBEⅠ, while the β-sheet and α-helix contents of CCBEⅡ were approximately 53.6% and 14.6%, respectively. The measurement errors of α-helix and β-sheet of CCBEs were less than 2% and 4%, respectively.

Key words: FT-IR spectroscopy, secondary structure prediction, chitosanase, cellulase, Trichoderma viride

CLC Number:

TS201.3

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