Orthogonal Array Design for Optimization of Enzymatic Extraction of Polysaccharides from Flos Lonicerae

Abstract

Abstract: n this study, an orthogonal array design was applied to optimize conditions for the hydrolysis of Flos Lonicerae by cellulase for polysaccharide extraction. The effects of enzyme concentration, temperature, pH and hydrolysis time on polysaccharide yield were explored. The optimum hydrolysis conditions for a considerably enhanced extraction yield of polysaccharide were determined as follows: 1.5% of enzyme concentration, 50 ℃, pH 5.0 and 50 min of extraction time.

Key words: cellulose, Flos Lonicerae, polysaccharide, enzymatic extraction

CLC Number:

Q814.1

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