Determination of Cordycepin in Fermentation Supernatant of Cordyceps militaris by HPLC and LC-MS/MS: A Comparison of Two Methods

doi:10.7506/spkx1002-6630-201314057

Abstract

Abstract:

Objective: To comparatively evaluate the feasibility of HPLC and LC-MS/MS for measuring cordycepin in
fermentation supernatant of Cordyceps militaris. Methods: The fermentation supernatants of 10 Cordyceps militaris strains were
purified by macroporous resin column chromatography. The purified samples obtained were analyzed separately by HPLC with
UV detection and LC-MS/MS. Results: The limit of detection (LOD) and limit of quantitation (LOQ) of HPLC were 13.6 μg/L and
53.6 μg/L, respectively, whereas those of LC-MS/MS were 2.1 μg/L and 7.8 μg/L, respectively. The retention time of cordycepin in HPLC
and LC-MS/MS was 22.7 min and 7.8 min, respectively, and the average recovery was 94.68% and 93.91%, respectively. Conclusion:
Both methods have high sensitivity, selectivity and simple operation, and can be used for qualitative and quantitative analysis.

Key words: HPLC, LC-MS/MS, cordyceps militaris fermentation supernatant, cordycepin

JIAN Li-ru，DU Shuang-tian. Determination of Cordycepin in Fermentation Supernatant of Cordyceps militaris by HPLC and LC-MS/MS: A Comparison of Two Methods[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201314057.

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Determination of Cordycepin in Fermentation Supernatant of Cordyceps militaris by HPLC and LC-MS/MS: A Comparison of Two Methods

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