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Effect of Casein Glycomacropeptide on Phagocytic Cells and Intestinal Mucosa Immune Cells in Mice

YE Lei, CHEN Qing-sen*, LI Wei, YAN Ya-li, ZHAO Pei, PANG Guang-chang, HU Zhi-he   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
    Tianjin University of Commerce, Tianjin 300134, China
  • Online:2014-05-15 Published:2014-05-19


Objective: Casein glycomacropeptide (CGMP) has many physiological functions and unique nutritional
properties. This study aimed to explore its effect on peritoneal immune cells and intestinal mucosal barrier in mice. Methods:
After being intragastrically given CGMP at doses of 30 and 120 μg/d, respectively, 96 female Kunming mice were tested
for phagocytic activity, intestinal intraepithelial lymphocytes (IEL) subpopulations and the number of IgA+ plasma cells in
the lamina propria by flow cytometry, hematoxylin and eosin (HE) staining and immunofluorescence, respectively. Results:
CGMP administration to mice resulted in a significant increase in phagocytic activity in a dose- and time-dependent manner.
Compared with the control group, CGMP administration also resulted in a significantly higher percentage of intraepithellal
lymphocytes (IEL) in the duodenum and a significantly greater number of IgA+ plasma cells in the lamina propria (P < 0.01).
Conclusion: CGMP can enhance phagocytic activity, induce intestinal mucosal immune response and enhance the intestinal
mucosal barrier function.

Key words: casein glycomacropeptide, intestinal mucosal barrier, phagocytic activity, intestinal intraepithelial lymphocytes;IgA+ plasma cells