FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (5): 135-142.doi: 10.7506/spkx1002-6630-20190226-195

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect and Underlying Mechanism of Mulberry Leaf Alkaloid on Improving D-Galactose-Induced Oxidative Damage in Mice

YANG Zhongmin, WANG Zuwen, HUANG Xianzhi, DING Xiaowen   

  1. (1. Chongqing Key Laboratory of Agricultural Products Processing and Store, National Demonstration Center for Experimental Food Science and Technology Education, College of Food Science, Southwest University, Chongqing 400716, China; 2. Science and Technology Department, Southwest University, Chongqing 400716, China)
  • Online:2020-03-15 Published:2020-03-23

Abstract: Objective: To investigate the improving effect of mulberry leaf alkaloid (MLA) on D-galactose (D-Gal)-induced oxidative damage in mice, and to illustrate its underlying mechanism from the perspective of the animal’s own antioxidant defense system. Methods: An oxidative stress model was induced by D-Gal. Ten normal mice were served as the normal control. The model mice were randomly divided into model control, positive control (glutathione at 200 mg/kg mb) and low-, medium- and high-dose MLA treatment groups (50, 100, and 200 mg/kg mb). After intragastric administration for 4 consecutive weeks, body mass, food intake and food utilization of the mice were measured. The levels of 8-iso-prostaglandin F2α (8-iso-PGF2α), protein carbonyl (PCO), advanced oxidation protein products (AOPP), 3-nitrotyrosine (3-NT), 8-hydroxy-2’-desoxyguanosine (8-OH-dG), 5-hydroxy-2’-deoxycotosine (5-OH-dC) in plasma were measured by enzyme-linked immunosorbent assay test kit. The activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), and the levels of metallothionein (MT) and thioredoxin (Trx), as well as total antioxidant capacity (TAC) in plasma were measured by using commercial test kits. Results: Compared with the model control group, body mass, food intake and food utilization of mice in the high-dose MLA group were significantly increased. The plasma contents of 8-iso-PGF2α, PCO, AOPP, 3-NT, 8-OH-dG, and 5-OH-dC in the medium- and high-dose MAL groups were significantly decreased (P < 0.05), and the activities of T-SOD and GSH-Px, the contents of MT and Trx, and TAC value were significantly increased (P < 0.05); all these effects were dose-dependent. In the high-dose group, the contents of 8-iso-PGF2α, PCO, AOPP, 3-NT, 8-OH-dG, and 5-OH-dC were significantly reduced by 83.46%, 35.43%, 61.70%, 63.65%, 65.02%, and 57.90%, respectively (P < 0.05). In addition, T-SOD, GSH-Px, MT, Trx, and TAC increased by 1.47, 2.96, 1.74, 1.37 and 1.63 times, respectively (P < 0.05), all of which reached the normal levels (P > 0.05). Conclusion: Mulberry leaf alkaloid can effectively improve lipid, DNA and protein oxidative damage in mice. The underlying mechanism may be related to regulating antioxidant enzyme activities and non-enzyme antioxidant contents, and enhancing the antioxidant defense system.

Key words: mulberry leaf alkaloid, D-galactose, biomacromolecule, oxidative damage

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