Abstract:

To establish a rapid analysis method for determination of common sweeteners such as acesulfame potassium, saccharin sodium, sodium cyclamate, aspavtame, and alitame in milk beverage by HPLC. After the pretreatments of dilution, protein precipitation, and high-speed centrifugation, the sample was separated by Symmetry C18 column with phosphate buffer (pH 5.0) and acetonitrile as mobile phase, eluted gradiently, and detected at the UV wavelength of 205 nm. The analysis of one sample costs 12 min, and all five sweeteners could be well separated. The average spike recoveries of the above five sweeteners were 86.0% to 102%, and the RSDs were 2.1% to 5.6% (n = 6). This method is simple, quick, and of good reproducibility and selectivity, therefore it is an effective way to determine sweeteners in milk drink.

Key words: sweetener, acesulfame potassium, saccharin sodium, sodium cyclamate, aspavtame, alitame, HPLC