Abstract:

In this study, horseradish peroxidase (HRP) was used to label anti-enrofloxacin antibody through a sodium periodate oxidation method. A one-step competitive direct enzyme-linked immunosorbent assay (ELISA) was developed for determining enrofloxacin in sea foods. The results showed that the titer of labeled antibody was was higher than 10000, and no significant cross-reactivity was observed with other fluoroquinolones and other groups of antibiotics. The detection limit was 10 μg/kg. For eel samples spiked with enrofloxacin from 10 to 40 μg/kg, the average recoveries were above 70% and the relative standard deviations were lower than 6%. The determination time by this method was shortened to less than 2 h due to pre-coating and blocking in microplates, which was about half of that by traditional two-step ELISA.

Key words: enrofloxacin, enzyme labeled antibody, immunoassay, sea food