Abstract:

In the present study, lactose-removed milk protein was hydrolyzed into peptides with chymotrypsin and trypsin together, and then after removing the substances with over 6000 D molecular weight by ultra-filtration, the residual hydrolysates were separated into 6 fractions (a, b, c, d, e, and f) by Sephadex G-15 gel chromatography. Kunming male mice were administered the above 6 fractions respectively by gavage for 28 days, and with the loaded swimming time as evaluation index, the best anti-fatigue peptide was chosen from the 6 fractions. Furthermore, through the determination of loaded swimming time, content of serum urea nitrogen after swimming, liver glycogen level, and serum lactate concentration before swimming of mice, its anti-fatigue ability was evaluated. The results showed that the fraction c is the best anti-fatigue peptide. It can significantly promote the loaded swimming time and liver glycogen level of mice, and reduce the contents of serum urea nitrogen and lactate of mice after swimming. It was concluded that the fraction c can improve the anti-fatigue ability of mice.

Key words: milk protein-derived peptide, anti-fatigue effect