Optimization of Culture Conditions for Post-biosynthesis of L-Glutamic Acid by Brevibacterium tianjinese S9114

doi:10.7506/spkx1002-6630-200904002

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (4): 40-43.doi: 10.7506/spkx1002-6630-200904002

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Optimization of Culture Conditions for Post-biosynthesis of L-Glutamic Acid by Brevibacterium tianjinese S9114

FENG Zhi-bin1，LIU Jin-jie1，WANG Dong-yang2，MIAO Jing1，QU Hui-ge1，
ZHANG Yu-xiang1，ZHANG Hua3，DING Juan1

(1. College of Life Science, Ludong University, Yantai 264025, China；2. Linghua Courmet Co. Ltd., Juancheng 272000,
China ；3. Yantai Nanshan University, Yantai 265706, China)

Received:2008-01-15 Revised:2008-05-04 Online:2009-02-15 Published:2010-12-29
Contact: FENG Zhi-bin1 E-mail:fengzhibin257@163.com

Abstract

Abstract:

The production process of L-glutamic acid by Brevibacterium tianjinese S9114 was investigated and the main affecting factors of post-biosynthesis of L-glutamic acid were investigated. The results showed that DO-control pulse fed-batch, and feeding of 2 μg/L biotin and 3 g/L KCl are beneficial for L-glutamic acid production. Under these optimal conditions, L-glutamic acid productivity reaches 148 g/L and conversion rate of glutamic acid to glucose reaches 60.5%.

Key words: L-glutamic acid, biotin, feeding method

CLC Number:

TQ922

FENG Zhi-bin1，LIU Jin-jie1，WANG Dong-yang2，MIAO Jing1，QU Hui-ge1，ZHANG Yu-xiang1，ZHANG Hua3，DING Juan1. Optimization of Culture Conditions for Post-biosynthesis of L-Glutamic Acid by Brevibacterium tianjinese S9114[J]. FOOD SCIENCE, 2009, 30(4): 40-43.

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Optimization of Culture Conditions for Post-biosynthesis of L-Glutamic Acid by Brevibacterium tianjinese S9114

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