Abstract:

A method was developed to clean-up and detect total patulin in of apple, hawthorn and tomato products using HPLCMS/ MS. Clear juice was diluted directly with acetonitrile and cleaned-up with MycoSep 228 multifunctional clean-up column. Cloudy juice or solid samples were pre-treated with pectinase, extracted with acetate ethyl, and then cleaned-up with MycoSep 228. Chromatographic separation was performed on a column Atlantis dC18 and determination was achieved by HPLC-MS/MS with ESI－(electronic spray ionization). The results demonstrated that the linear ranges are from 1.0 μg/kg to 200 μg/kg with the detection limit of 2.0 μg/kg. The average recoveries and the relative standard deviations range 76.0% to 95.3% and 5.4% to 11.2%. The method is simple, rapid, accurate and suitable for the quantification and confirmation determination of patulin in apples, hawthorn, tomato products.

Key words: patulin, HPLC-MS/MS, ESI, mycotoxin multifunctional clean-up column, apple products, hawthorn products, tomato products