FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22 ): 119-121.doi: 10.7506/spkx1002-6300-200922025

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Use of Xylanase and Cellulase for Preparation of Soluble Dietary Fiber from Corn Bran

HOU Chuan-wei,WEI Shu-xin,WANG An-jian   

  1. (Institute of Agricultural Products Processing, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
  • Received:2009-05-24 Online:2009-11-15 Published:2010-12-29
  • Contact: HOU Chuan-wei, E-mail:nkyhcw@yahoo.com.cn

Abstract:

The insoluble fiber in corn bran by-product after ultrasonic-assisted extraction of natural soluble dietary fiber was further hydrolyzed using xylanase and cellulase for preparation of soluble dietary fiber. By conducting single factor experiments and orthogonal tests, conditions for the enzymatic hydrolysis were optimized as follows: cellulase 40 mg/g substrate, xylanase 40 mg/g substrate, solid-to-liquid ratio 1:14, extraction time 90 minutes. Under such conditions, the yield of SDF was 5.96%.

Key words: insoluble corn bran, xylanase, cellulase, soluble dietary fiber

CLC Number: