Abstract: Aloin was extracted from dry aloe powder with methanol, further purified by solid phase extraction column, and then determined by high performance liquid chromatograph (HPLC). The HPLC operation conditions were as follows: C18 column (4.6 mm×150 mm, 5μm), methnaol-1% acetic acid water (1:1, V/V) as mobile phase, and UV detector wavelength 359 nm. The quantitative and qualitative analyses were performed with external standard method. The results of determination showed that there is a good linear calibration curve in the range from 0 to 100 mg/L (r=0.9999) with relative standard deviations of 2.5% to 3.2% (n=6). The recovery rates of spiked aloin range from 98.1% to 101%, and the detection limit is 0.02 mg/kg. This method is rapid, sensitive, simple, accurate and repeatable.

Key words: dry aloe powder, aloin, solid phase extraction, high performance liquid chromatography