Quantitative Determination of Alkaloids in Areca Nut and Its Products by RP-IP-HPLC

Abstract

Abstract: A method was developed for quantitative dtermination of arecoline and arecaidine in areca nut and its products by reversed-phase ion-pair high performance liquid chromatography (RP-IP-HPLC). Chromatographic separation was performed on Hypersil BDS C18 (250×4.6 mm, 5μm) column using a mixture (water: methanol: phosphoric acid: SDS=55 ml : 45 ml : 0.1 ml :1 g) as mobile phase with flow rate 1.0 ml/min and using SPD-20A ultraviolet detection at 215 nm. Arecoline and arecaidine are eluted within 25 min with satisfactory separation. Relative standard deviation of 0.84%～1.56% and average recovery of 96.77%～100.23% for the method are achieved. The contents of alkaloids in different areca nuts and their products were determinded by this method.

Key words: HPLC, areca nut, arecoline, arecaidine

ZHANG Chun-Jiang, 吕Fei-Jie , TAI Jian-Xiang, WANG Zhu-Nian, FU Qin. Quantitative Determination of Alkaloids in Areca Nut and Its Products by RP-IP-HPLC[J]. FOOD SCIENCE, 2008, 29(7): 345-347.

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Quantitative Determination of Alkaloids in Areca Nut and Its Products by RP-IP-HPLC

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