FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (2): 210-212.

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Preliminary Study on Deletion of Saccharomyces cerevisiae Alcohol Dehydrogeniase I Gene

GE  Jing-Ping, SONG  Gang, ZONG  Xiang, LING  Hong-Zhi, CAI  Bai-Yan, LIU  Song-Mei, PING  Wen-Xiang   

  1. Heilongjiang Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China
  • Online:2008-02-15 Published:2011-08-24

Abstract: The main purpose of this research is to construct a low alcohol producing strain according to the alcohol metabolic pathway of Saccharomyces cerevisiae, so as to satisfy the people who prefer to drink low-alcohol beer. Hygromycin B resistant gene was used to screen mutants with adh I gene knocked out. After Hygromycin B resistant gene was amplified with primers L1 and L2 (the flanking fragments were complement with Saccharomyces cerevisiae gene), it was transformed into yeast HDY-01 by LiAc method and the alcohol dehydrogenase I (ADH I) in Saccharomyces cerevisiae was deleted through homologous recombination. A transformant was obtained with low ADH I activity. The fermentation tests showed that the average alcohol content of the transformant is 1.8%(V/V), 65% lower than the origin one. The alcohol metabolic pathway in this transformant isi nterfered.

Key words: Saccharomyces cerevisiae, gene deletion, alcohol dehydrogenase I (ADH I)