Abstract: AMP(2-amino-2-methylpropanol)was developed as chemical modifying reagent of fatty acids so as to modify carboxyl group into nitrogen-containing heterocycle which effectively suppressed the migration of double bonds of the alkenyl under the electron impact source.13 fatty acids were identified according to the analysis of GC-EIMS and the position of double bonds of the fatty acids in hippocampus was located by analyzing electron impact spectra of the chemical products modified by AMP. The contents of the unsaturated fatty acids and the polyunsaturated fatty acids are 75.15% and 26.17%, respectively, and the main components of the polyunsaturated fatty acids are 9,12-linoleic acid,9,12,15-linolenic acid and 4,7,10,13,16,19-docosahexenoica cidr espectively.

Key words: hippocampus, fatty acids, 2-amino-2-methylpropanol, chemical modification, gas chromatography-electron impact mass spectrometry