Abstract: The 3-hemiglutarate-deoxynivalenol (3-HG-DON) was prepared by protection of the C7- and C15-hydroxyls with a cyclic boronate ester, esterified with glutarate at the C3-hydroxyl, and then the boronate ester removal as tested by TLC (thin layer chromatography) and UV spectrometer. ESI-MS. 3-HG-DON was activated by active ester method, and conjugated to ovalbumin (OVA) as DON detecting antigen (DON-HG-OVA). DON was activated by 1,1-Dicyclohexylcarbodiimide (CDI), and conjugated to modify bovine serum albumin (MBSA) as immunogen (DON-MBSA). The titer of anti-DON antibodies in one BLAB/c mice serum, immunized by DON-MBSA, is about 1:25600, in which antibodies binding to DON-HG-OVA can be inhibited by free DON.

Key words: deoxynivalnol, artificial antigen