Determination of Volatile Fatty Acids by Hollow Fiber Supported Liquid Membrane Extraction Coupled with Gas Chromatography

doi:10.7506/spkx1002-6630-201224039

Abstract

Abstract: A method for the determination of volatile fatty acids (VFAs) in meat by hollow fiber supported liquid membrane extraction (HF-SLME) coupled with gas chromatography (GC) was developed and validated. HF-SLME was employed to selectively enrich VFAs from the acidified aqueous supernatant of meat homogenate. The extract was further acidified with HCl and analyzed by GC. The proposed method provided high linearity (r≥0.9261), low detection limits (0.03–0.16 μmol/L), acceptable spiked recovery (71.0%–123.1%), and reasonable intra-assay (7.82%–10.28%) and inter-assay (10.01%–15.96%) precision. Total VFAs content in livestock meat (725.30–845.23 mg/kg) are much higher than those from poultry (192.82–282.39 mg/kg). Total VFAs content in pork was negatively correlated with its pH (r=0.7592) and total volatile basic nitrogen (TVB-N) (r = 0.7890). These observations suggest that total VFAs may an indicator of the freshness of meat.

Key words: volatile fatty acids (VFAs), hollow fiber supported liquid membrane extraction (HF-SLME), meat freshness, gas chromatography (GC)

CLC Number:

TS201.21

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