Optimization of Extraction Process for Chlorophyll from Young Barley Grasses Based on Response Surface Methodology

doi:10.7506/spkx1002-6630-201402014

FOOD SCIENCE ›› 2014, Vol. 35 ›› Issue (2): 75-80.doi: 10.7506/spkx1002-6630-201402014

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Optimization of Extraction Process for Chlorophyll from Young Barley Grasses Based on Response Surface Methodology

ZHANG Hui1,2, ZHANG Na-na3, MA Li1, TANG Jian3, QIAO Yong-jin2,*

1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;
2. Agricultural Products Storage and Processing Research Center, Shanghai Academy of Agricultural Science, Shanghai 201403,
China; 3. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China

Received:2013-05-09 Revised:2013-12-23 Online:2014-01-25 Published:2014-02-19
Contact: QIAO Yong-jin E-mail:nd_zh@sina.com

Abstract

Abstract:

The extraction of chlorophyll from young barley grasses with aqueous ethanol as extraction solvent was
optimized. Significant variables that influence extraction efficiency were selected by Plackett-Burman experimental design
and optimized by central composite experimental design. Solid-to-solvent ratio, ethanol concentration and extraction time
were identified as main influencing parameters. The optimal extraction conditions were found to be 31:1, 92% and 112 min.
Under these conditions, the observed yield of chlorophyll was 13.646 mg/g, approximating to the predicted value of
13.622 mg/g. It can be concluded that response surface methodology is applicable to optimize the parameters for extraction
of chlorophyll from young barley grasses and the optimized extraction process parameters can provide a reference for
industrial production of chlorophyll from young barley grasses.

Key words: Plackett-Burman design, central composite design, chlorophyll of young barley grasses, optimization of extraction process

CLC Number:

R284.2

ZHANG Hui1,2, ZHANG Na-na3, MA Li1, TANG Jian3, QIAO Yong-jin2,*. Optimization of Extraction Process for Chlorophyll from Young Barley Grasses Based on Response Surface Methodology[J]. FOOD SCIENCE, 2014, 35(2): 75-80.

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Optimization of Extraction Process for Chlorophyll from Young Barley Grasses Based on Response Surface Methodology

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