FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (11): 194-200.doi: 10.7506/spkx1002-6630-20190603-019

• Nutrition & Hygiene • Previous Articles     Next Articles

Protective Effect of Betulinic Acid on Intestinal Oxidative Damage Induced by T-2 Toxin in Mice

HUANG Chenglong, ZHU Lijuan, YI Xianglian, HUANG Lin, OU Zhaoping, MA Yurong, WU Jing, LI Rongfang, YI Jin’e   

  1. (1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China; 2. Hunan Co-innovation Center of Animal Production Safety, Changsha 410128, China; 3. Hunan Engineering Research Center of Livestock and Poultry Health Care, Changsha 410128, China)
  • Online:2020-06-15 Published:2020-06-22

Abstract: In order to explore the protective effect of betulinic acid (BA) on T-2 toxin-induced intestinal oxidative damage, 60 healthy male mice were randomly divided into 6 equal groups: control, T-2 toxin, low-, medium- and high-dose BA (0.25, 0.5 and 1 mg/kg mb) plus T-2, and vitamin E plus T-2. After BA was administered for 14 days, the levels of immunoglobulin (Ig)M, IgG, and diamine oxidase (DAO) in serum were detected by enzyme-linked immunosorbent assay, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px), and the contents of malondialdehyde (MDA) and glutathione (GSH) in the duodenum, jejunum and ileum were determined. Morphological changes in the jejunum were observed by hematoxylin-eosin (H & E) staining. The results showed that BA enhanced the levels of IgG and IgM, and reduced the activity of DAO in the serum. Meanwhile, it alleviated the decrease of CAT and GSH-Px activities caused by T-2 toxin in the intestine, increased GSH content, and decreased MDA content. Therefore, BA has a protective effect on intestinal oxidative damage induced by T-2 toxin in mice by increasing humoral immunity function, improving intestinal mucosal barrier function and enhancing intestinal antioxidant capacity.

Key words: betulinic acid, T-2 toxin, intestinal mucosa, oxidative damage

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