Screening and identification of in vitro anti-allergic active probiotics based on the degranulation inhibition of RBL-2H3 cells

Abstract

Abstract: To investigate the anti-allergic activity of lactic acid bacteria, this paper screened probiotics with anti-allergic activity by traditional isolation technique of lactic acid bacteria and in vitro evaluation of probiotics, combined with RBL-2H3 cell degranulation inhibition test. The results showed that the four strains isolated from fermented vegetables exhibited good probiotic properties and safety; the selected four strains showed high inhibition of β-Hexosaminidase release from RBL-2H3 cell degranulation, and the four strains showed high inhibition of Histamine, Histamine release from RBL-2H3 cell degranulation, Histamine, Histamine release from RBL-2H3 cell degranulation, and Histamine release from RBL-2H3 cell degranulation, The four strains were identified by 16S rDNA sequencing as Lactobacillus plantarum, Lactobacillus fermentum and Lactobacillus paracasei.

Key words: probiotics, RBL-2H3 cells, probiotic properties, degranulation, anti-allergy

CLC Number:

TS201.3

MA Ding Qin Shuangxia. Screening and identification of in vitro anti-allergic active probiotics based on the degranulation inhibition of RBL-2H3 cells[J]. FOOD SCIENCE.

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