Optimization of Enzymatic Polysaccharide Extraction from Acaudina molpadioides Semper by Response Surface Methodology

doi:10.7506/spkx1002-6630-201020013

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (20): 61-66.doi: 10.7506/spkx1002-6630-201020013

• Processing Technology • Previous Articles Next Articles

Optimization of Enzymatic Polysaccharide Extraction from Acaudina molpadioides Semper by Response Surface Methodology

XU Lu，YE Li-bin，YU Ping，LI Jian-rong*

Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology,
Zhejiang Gongshang University, Hangzhou 310035, China

Received:2010-01-11 Online:2010-10-25 Published:2010-12-29
Contact: LI Jian-rong* E-mail:lijianrong@zjgsu.edu.cn

Abstract

Abstract:

Papain, pepsin and trypsin were chosen to conduct the single enzyme hydrolysis of Acaudina molpadioides Semper for polysaccharide extraction, and pepsin was the selected enzyme due to the highest polysaccharide yield. The hydrolysis of Acaudina molpadioides Semper with pepsin was optimized by response surface methodology in order to improve polysaccharide yield. The optimal conditions for pepsin hydrolysis were found to be: enzyme load, 0.95%; hydrolysis temperature, 56 ℃; initial pH, 1; and hydrolysis duration, 6 h. An experimental polysaccharide yield of 1.65% was obtained under these conditions, which basically accorded with the model predicted value of 1.59%.

Key words: Acaudina molpadioides Semper, polysaccharide, enzymoysis, response surface methodology

CLC Number:

TQ929.2

XU Lu，YE Li-bin，YU Ping，LI Jian-rong*. Optimization of Enzymatic Polysaccharide Extraction from Acaudina molpadioides Semper by Response Surface Methodology[J]. FOOD SCIENCE, 2010, 31(20): 61-66.

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Optimization of Enzymatic Polysaccharide Extraction from Acaudina molpadioides Semper by Response Surface Methodology

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