Partitional Behavior of Ficin in PEG/(NH4)2SO4 Two-phase Aqueous System

doi:10.7506/spkx1002-6630-201019012

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (19): 67-70.doi: 10.7506/spkx1002-6630-201019012

• Basic Research • Previous Articles Next Articles

Partitional Behavior of Ficin in PEG/(NH4)2SO4 Two-phase Aqueous System

FENG Zi-li1，MA Na2

1. Bio-resources Key Laboratory of Shaanxi Province, Shaanxi University of Technology, Hanzhong 723001, China；
2. Department of Physics, Shaanxi University of Technology, Hanzhong 723001, China

Received:2009-10-11 Revised:2010-07-23 Online:2010-10-15 Published:2010-12-29
Contact: FENG Zi-li E-mail:fengzili2008@163.com

Abstract

Abstract:

Aqueous two-phase system (ATPS) was a new and cheap purification method for ficin. The partition of Ficin in polyethylene glycol and ammonium sulfate aqueous two-phase systems was investigated. The effects of PEG molecular weight, polyethylene glycol concentration and ammonium sulfate concentration, pH and temperature on ficin partition coefficient were also explored. Results exhibited that ficin partition coefficient in two-phase aqueous system at the conditions of 20% PEG1000, 19% (NH4)2SO4, pH 6 and temperature at 25 ℃ was 3.6.

Key words: ficin, two-phase aqueous system, partition coefficient K

CLC Number:

Q814.1

FENG Zi-li1，MA Na2. Partitional Behavior of Ficin in PEG/(NH4)2SO4 Two-phase Aqueous System[J]. FOOD SCIENCE, 2010, 31(19): 67-70.

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Partitional Behavior of Ficin in PEG/(NH4)2SO4 Two-phase Aqueous System

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