关键词: 小米多肽, 制备, 抗氧化

Abstract:

Millet peptides were prepared by means of alcalase hydrolysis, and the optimum enzymatic hydrolysis parameters were determined by single-factor test and orthogonal design. Then their scavenging activity to surperoxide anion radical and hydroxyl radical were detected by nitroblue tetrazolium (NBT) test and deoxyribose method, and the extent of erythrocatalysis and the formation of malondialdehyde (MDA) in liver mitochondria were detected by spectrophotometric method. The results showed that the optimum preparation conditions for millet peptides are hydrolysis with 5% of concentration ratio of enzyme to substrate at pH 8.5 and 40 ℃ for 3.5 h. The scavenging activity of millet peptides to DPPH radical, superoxide anion radical (O2·) and hydroxyl radical (·OH) are 68.93%, 40.06% and 48.63%, respectively. Moreover, the millet peptides can effectively inhibit the hemolysis of red blood cells and the damage of mitochondria. Therefore, they have significant antioxidation.

Key words: millet peptides, preparation, antioxidation