食品科学 ›› 2013, Vol. 34 ›› Issue (6): 64-67.doi: 10.7506/spkx1002-6630-201306014

• 工艺技术 • 上一篇    下一篇

正交试验优化纤维素酶法提取牛蒡根皮中绿原酸工艺

林春梅,周鸣谦   

  1. 淮海工学院食品科学与工程学院,江苏 连云港 222005
  • 收稿日期:2011-12-19 修回日期:2013-01-31 出版日期:2013-03-25 发布日期:2013-03-01
  • 基金资助:
    江苏省产学研联合创新资金计划

Optimization by Orthogonal Array Design of Extraction of Chlorogenic Acid from Burdock Root Peels

LIN Chun-mei,ZHOU Ming-qian   

  1. College of Food Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China
  • Received:2011-12-19 Revised:2013-01-31 Online:2013-03-25 Published:2013-03-01

摘要: 目的:研究纤维素酶法提取牛蒡根皮中绿原酸的提取工艺。方法:通过单因素试验探讨纤维素酶用量、料液比、酶解温度、酶解时间、pH值对绿原酸提取率的影响,并通过正交试验对影响绿原酸提取率的参数进行优化。结果表明牛蒡根皮中绿原酸提取的最佳工艺条件为纤维素酶用量4mL、酶解温度60℃、酶解时间1.5h、pH6.0、料液比1:20,此条件下绿原酸提取率为1.45%。

关键词: 牛蒡, 绿原酸, 提取

Abstract: Objective: To optimize extraction process of chlorogenic acid in burdock root peels by cellulose methods. Methods: The effects of enzyme dosage, material-liquid ratio, extraction temperature, extraction duration and extraction pH on the yield of chlorogenic acid from burdock root peels by single factor and orthogonal tests were explored. Results: The optimal extraction process included cellulose addition amount of 4 mL, extraction temperature of 60 ℃, extraction time of 1.5 h, extraction pH 6.0, and material-liquid ratio of 1:20. Under the optimal extraction conditions, the maximum yield of chlorogenic acid was 1.45%.

Key words: burdock, chlorogenic acid, extraction

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