食品科学 ›› 2011, Vol. 32 ›› Issue (24): 60-63.doi: 10.7506/spkx1002-6630-201124013

• 工艺技术 • 上一篇    下一篇

茜草总黄酮提取工艺研究

田春莲1,蒋凤开2   

  1. 1.吉首大学 林产化工工程湖南省重点实验室 2.吉首大学生物资源与环境科学学院
  • 出版日期:2011-12-25 发布日期:2011-12-29
  • 基金资助:
    湖南省科技计划项目(2010TP4007-1);林产化工工程湖南省重点实验室开放基金项目(JDZ200906); 湖南省高校“林产资源化学与林化产品开发”科技创新团队支持计划项目(湘教通(2010)212 号)

Optimization of Extraction Process for Total Flavonoids from Madder Root

TIAN Chun-lian1,JIANG Feng-kai2   

  1. (1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China; 2. College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China)
  • Online:2011-12-25 Published:2011-12-29

摘要: 研究茜草总黄酮的提取工艺条件。采用热浸提法提取茜草总黄酮,硝酸铝显色法测定总黄酮提取率。通过单因素试验研究乙醇体积分数、料液比、提取温度、提取时间对黄酮提取率的影响,通过正交试验进一步优化提取工艺条件。单因素试验结果显示,乙醇体积分数50%、提取温度70℃、提取时间2.5h、料液比1:40(g/mL)条件下茜草总黄酮提取率较高;正交试验结果表明:茜草总黄酮的最佳提取工艺为60%、乙醇溶液提取2h、提取温度70℃、料液比1:40(g/mL),此条件下茜草总黄酮提取率为1.67%。本工艺简单可行,有较好的参考价值。

关键词: 茜草, 总黄酮, 提取

Abstract: This study was carried out to optimize the hot water extraction of total flavonoids from madder root. The effects of 4 process parameters including ethanol concentration, material-to-liquid ratio, temperature and extraction duration on extraction efficiencywere investigated by one-factor-at-a-time method and orthogonal array design. The results form the one-factor-at-a-time experiments showed that the optimal extraction conditions were 50% aqueous ethanol as extraction solvent at a material-to-liquid ratio of 1:40 (g/mL), temperature 70℃ and extraction duration 2.5 h. The results from the subsequent orthogonal array design experiments indicated that the highest extraction efficiency (1.67%) for madder root was achieved after 2 h of extraction with 60% aqueous ethanol at 70 ℃ and a material-to-liquid ratio of 1:40 (g/mL). In conclusion, the optimized extraction process is simple and feasible and has great reference value.

Key words: madder, total flavanoids, extraction

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