余甘子冻干粉对急、慢性高尿酸血症小鼠的降尿酸及肝肾保护作用

摘要/Abstract

摘要： 目的：探究余甘子冻干粉对急性和慢性高尿酸血症（Hyperuricemia，HUA）小鼠的降尿酸效果及对肝肾的保护作用。方法：分别采用腹腔注射次黄嘌呤和氧嗪酸钾建立急性和慢性HUA小鼠模型，灌胃75、150、300 mg/kg的余甘子冻干粉作为干预物质，检测小鼠血清尿酸（uric acid，UA）、尿素氮（urea nitrogen，BUN）、肌酐（creatinine，CRE）水平和天门冬氨酸氨基转氨酶（Aspartate aminotransferase，AST）、谷氨酸氨基转氨酶（Alanine aminotransferase，ALT）含量以及血清和肝脏组织中的黄嘌呤氧化酶（Xanthine oxidase，XOD）和腺苷脱氨酶(Adenosine deaminase，ADA)活性，计算肝肾系数，HE染色法观察肝肾组织的病理学特征。结果：与模型组相比，150、300 mg/kg的余甘子冻干粉可显著降低急性HUA小鼠肝脏系数和肾脏系数（P<0.001），下调血清UA（P<0.001，P<0.01）、BUN及CRE（P<0.01，P<0.001）、AST（P<0.001）和ALT（P<0.001，P<0.05）水平，抑制血清和肝脏中XOD和ADA活性（P<0.001）；75、150、300 mg/kg的余甘子冻干粉能降低慢性HUA小鼠肝脏系数（P<0.05或P<0.001）和肾脏系数（P<0.001），下调血清UA及BUN（P<0.001）、CRE（P<0.05或P<0.001）、AST（P<0.01或P<0.001）和ALT（P<0.001）水平，抑制血清和肝脏中XOD（P<0.001）和ADA（P<0.05或P<0.001）活性。病理结果提示：余甘子冻干粉可改善急性和慢性HUA小鼠肝脏和肾脏的病理变化。结论：余甘子冻干粉能有效降低急性和慢性HUA小鼠的血清尿酸水平，可能与抑制血清和肝脏中的XOD和ADA活性及促进肾脏尿酸排泄有关，同时具有明显肾脏保护作用及发挥了一定的肝脏保护作用。

关键词: 余甘子, 高尿酸血症, 黄嘌呤氧化酶（XOD）, 腺苷脱氨酶（ADA）, 肝肾保护

Abstract: Objective: To explore the uric acid-lowering and hepatorenal protective effects of Phyllanthus emblica L. freeze-dried powder (PEFP) in mice with acute and chronic hyperuricemia. Methods: Acute and chronic hyperuricemia mouse models were established by intraperitoneal injection of hypoxanthine and potassium oxazinate respectively. Meanwhile, PEFP at 75, 150 or 300 mg/kg were given to the mice by gavage as intervention substances. The levels of uric acid (UA), creatinine (CRE), urea nitrogen (BUN), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, the activities of xanthine oxidase (XOD) and adenosine deaminase (ADA) in serum and hepar were detected. The hepatic coefficient and renal coefficient of mice were calculated, and the pathological characteristics of liver and kidney were observed by HE staining. Results: Compared with the model group, 150 or 300 mg/kg PEFP could reduce the hepatic coefficient and renal coefficient (P<0.001), lower serum UA(P<0.01, P<0.001), BUN and CRE(P<0.01, P<0.001), AST(P<0.001) and ALT content(P<0.05, P<0.001), inhibit serum and hepar XOD and ADA activities (P<0.001) in acute hyperuricemia mice. Meanwhile, 75, 150 or 300 mg/kg PEFP can reduce the hepatic coefficient (P<0.05 or P<0.001) and renal coefficient (P<0.001), lower serum UA and BUN(P<0.001), CRE(P<0.05 or P<0.001), AST(P<0.01 or P<0.001)and ALT content(P<0.001), inhibit serum and hepar XOD (P<0.001) and ADA activities (P<0.05 or P<0.001) in chronic hyperuricemia mice. The pathological results suggested that PEFP could ameliorate the pathological changes of liver and kidney in acute and chronic hyperuricemia mice. Conclusion: Phyllanthus emblica L. freeze-dried powder can effectively decrease the serum uric acid level in acute and chronic hyperuricemia mice. The uric acid-lowering effect of which might be related to the inhibition of XOD and ADA activities in serum and hepar and the promotion of uric acid excretion in kidney. Meanwhile, Phyllanthus emblica L. freeze-dried powder has obvious renal protective effect and has a certain hepatic protective effect on acute and chronic hyperuricemia mice.

Key words: Phyllanthus emblica L., Hyperuricemia, Xanthine oxidase, Adenosine deaminase, Hepatorenal protection

中图分类号:

TS201.4

李瑞婷何维宋贺谢小亭吴杰姜燕. 余甘子冻干粉对急、慢性高尿酸血症小鼠的降尿酸及肝肾保护作用[J]. 食品科学, 0, (): 0-0.

瑞婷李 Wei He. Effects of freeze-dried powder of Phyllanthus emblica L. on lowering uric acid and protecting liver and kidney in mice with acute and chronic hyperuricemia[J]. FOOD SCIENCE, 0, (): 0-0.

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