粟米油中脂肪酸及其主成分亚油酸的分析

doi:10.7506/spkx1002-6630-201022086

食品科学 ›› 2010, Vol. 31 ›› Issue (22): 391-393.doi: 10.7506/spkx1002-6630-201022086

粟米油中脂肪酸及其主成分亚油酸的分析

侯冬岩，回瑞华，李铁纯，刘晓媛

鞍山师范学院化学系

收稿日期:2010-03-31 出版日期:2010-11-25 发布日期:2010-12-29
通讯作者: 回瑞华 E-mail:ruihuahui@163.com
基金资助:
辽宁省教育厅科学技术基金项目(20331079)

Determination of Fatty Acids Composition and Octadecadienoic Acid in Corn Embryo Oil

HOU Dong-yan，HUI Rui-hua，LI Tie-chun，LIU Xiao-yuan

Department of Chemistry, Anshan Normal University, Anshan 114007, China

Received:2010-03-31 Online:2010-11-25 Published:2010-12-29
Contact: HUI Rui-hua E-mail:ruihuahui@163.com

摘要/Abstract

摘要：

高效液相色谱法对粟米油脂肪酸的组成和主成分亚油酸进行定量分析。采用索氏提取法从玉米胚芽中提取粟米油，并对粟米油中脂肪酸进行甲酯化处理，利用气相色谱- 质谱法对其脂肪酸组成进行鉴定。色谱柱：KromasilC18；流动相为异丙醇:乙腈=35:65；柱温：25℃；205nm 检测波长条件下对亚油酸进行测定。结果表明：由粟米油中分离鉴定出8 种脂肪酸，脂肪酸的主要组成是不饱和脂肪酸，不饱和脂肪酸占74.94%，主要成分为亚油酸和油酸，其中亚油酸为46.33%，油酸为28.01%。亚油酸变异系数小于1.35%，回收率为98.8%～109.1%。本方法简便、准确，适合粟米油中亚油酸含量的分析。

关键词: 粟米油, 脂肪酸, 气相色谱-质谱联用, 高效液相色谱, 亚油酸

Abstract:

The fatty acid composition and the contents of octadecadienoic acid in corn embryo oil were determined by using gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC), respectively. The corn embryo oil was extracted by soxhlet extraction, then the fatty oil obtained was esterified by sodium hydroxide-methanol. After purification, the methylated fatty acids were subjected to GC-MS analysis. Totally 8 fatty acids were identified in the corn embryo oil, and 74.94% fatty acids were unsaturated fatty acids. Moreover, the unsaturated fatty acids consisted of octadecadienoic acid (46.33%) and the octadecenoic acid (28.01%). In the following, the content of the octadecadienoic acid in corn embryo oil was determinated by HPLC. The column was Kromasil C18. The mobile phase consisted of iso-propyl alcohol and acetonitrile (35:65). The column temperature was 25 ℃, and detected at 205 nm. The regression equation obtained was y = 1×106x +0.7×106, r =0.9999. The coefficient of variation was less than 1.35%, and the recovery of the method was 98.8%－109.1%. The result indicates that the method is simple , accurate and feasible for determination of octadecadienoic acid in corn embryo oil.

Key words: corn embryo oil, fatty acid, GC-MS, HPLC, octadecadienoic acid

中图分类号:

侯冬岩，回瑞华，李铁纯，刘晓媛. 粟米油中脂肪酸及其主成分亚油酸的分析[J]. 食品科学, 2010, 31(22): 391-393.

HOU Dong-yan，HUI Rui-hua，LI Tie-chun，LIU Xiao-yuan. Determination of Fatty Acids Composition and Octadecadienoic Acid in Corn Embryo Oil[J]. FOOD SCIENCE, 2010, 31(22): 391-393.

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粟米油中脂肪酸及其主成分亚油酸的分析

Determination of Fatty Acids Composition and Octadecadienoic Acid in Corn Embryo Oil

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