食品科学 ›› 2010, Vol. 31 ›› Issue (19): 426-429.doi: 10.7506/spkx1002-6630-201019094

• 专题论述 • 上一篇    下一篇

食物过敏原蛋白的定量检测方法

闫 飞1,廖志勇2,吴志华1,*,陈红兵1   

  1. 1.南昌大学 食品科学与技术国家重点实验室,中德联合研究院 2.温州大学生命与环境科学学院
  • 收稿日期:2010-06-30 修回日期:2010-09-16 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 吴志华 E-mail:wuzhihua@ncu.edu.cn
  • 基金资助:

    江西省自然科学基金项目(2007GQN2001);江西省教育厅科技计划项目(赣教技字[GJJ08052]号)

A Review of Quantitative Detection of Food Allergens

YAN Fei1,LIAO Zhi-yong2,WU Zhi-hua1,*,CHEN Hong-bing1   

  1. 1. State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University,
    Nanchang 330047, China;2. College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China
  • Received:2010-06-30 Revised:2010-09-16 Online:2010-10-15 Published:2010-12-29
  • Contact: WU Zhi-hua E-mail:wuzhihua@ncu.edu.cn

摘要:

食物过敏现象的日益严重凸显食物过敏原蛋白定量检测的重要性。目前对食物过敏原蛋白进行定量检测的方法多是基于单个蛋白的间接测定。随着质谱分析方法的进步,定量蛋白质组学技术已开始并将广泛应用于复杂食品混合物中微量过敏原的直接定量检测。本文分别对基于单个蛋白和蛋白质组学的食物过敏原蛋白的定量测定方法进行综述。

关键词: 定量, 过敏原, 质谱, 蛋白质组学

Abstract:

The increasing phenomena of food allergy have highlighted the importance of quantitative analysis of food allergens. Currently, most methods for the quantitative detection of food allergens are indirect methods based on a single protein. Due to the development of mass spectrometry, quantitative proteomics is being widely used for the direct quantitative detection of trace allergens in complex food mixtures. In this paper, the research progress in quantitative detection methods for food allergens is discussed.

Key words: quantification, food allergen, mass spectrometry (MS), proteomics

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