食品科学 ›› 2010, Vol. 31 ›› Issue (13): 60-64.doi: 10.7506/spkx1002-6630-201013015

• 基础研究 • 上一篇    下一篇

野生山毛豆蛋白制备及体外消化模拟研究

于立梅,于 新,曾晓房,杨凤卿   

  1. 仲恺农业工程学院轻工与食品学院
  • 收稿日期:2009-12-07 出版日期:2010-07-01 发布日期:2010-12-29
  • 通讯作者: 于立梅 E-mail:biyingwang2003@163.com
  • 基金资助:

    广东省科技计划资助项目(2008B030302001)

Preparation and in vitro Digestibility of Tephrosia candida Protein

YU Li-mei,YU Xin,ZENG Xiao-fang,YANG Feng-qing   

  1. College of Light Industry and Food Science, Zhongkai University of Agricultural and Engineering, Guangzhou 510225, China
  • Received:2009-12-07 Online:2010-07-01 Published:2010-12-29
  • Contact: YU Li-mei E-mail:biyingwang2003@163.com

摘要:

通过碱提酸沉法制备野生山毛豆蛋白,并模拟人体消化环境,采用胃蛋白酶、胰蛋白酶两步消化法分析山毛豆蛋白的消化行为。结果表明:山毛豆中蛋白质含量约为(47.12 ± 0.35)%,大豆中蛋白质含量约为(44.17 ±0.48)%,山毛豆含有18 种氨基酸,其中包括人体必需的8 种氨基酸,从非必需氨酸酸来看,山毛豆蛋白与大豆蛋白有相似的氨基酸组分,氨基酸平衡状况较好,接近参考蛋白模式;山毛豆的体外消化率为(84.86 ± 0.75)%,大豆的体外消化率为(91.71 ± 0.88)%,山毛豆酶解液中的总氨基酸含量比大豆的略低,但山毛豆酶解液中甘氨酸和色氨酸含量比大豆酶解液高很多,游离氨基酸(FAA)含量比大豆的略高。

关键词: 山毛豆, 蛋白质, 消化率

Abstract:

Tephrosia candida protein (TCP) was obtained through alkaline extraction and acid precipitation. Its digestion behavior was explored through two-step digestion using pepsin and trypsin in mimic digestion environment. Results exhibited that the content of soybean protein was (44.17 ± 0.48)%, the content of TCP was (47.12 ± 0.35)% and this protein was composed of 18 kinds of free amino acids including 8 kinds of essential acids. However, similar amino acid pattern between TCP and soybean protein was observed. The digestibility of TCP was (84.86 ± 0.75)%, while the digestibility of soybean protein was (91.71 ± 0.88)%. The content of total amino acids of TCP hydrolysates was lower than that of soybean hydrolysates, but the contents of some of the tested free amino acids were higher than those of their counterparts in TCP hydrolysates.

Key words: Tephrosia candida, protein, digestibility

中图分类号: