Abstract:

The microwave-assisted extraction of polysaccharides from the submerged fermentation broth of Grifola
frondosa and the anti-tumor activity of the extracted polysaccharides was evaluated in a tumor-bearing mouse model.
Different extractions parameters including solvent pH, microwave power, treatment time and extraction time were optimized
using response surface methodology. The optimum extraction conditions that provided maximum yield of polysaccharides
of 7.19 g/L, which was 24.39% higher than before the optimization were found as follows: solvent pH of 7.0, microwave
power of 570 W, treatment time of 4.5 min and hot water extraction time of 2 h. Furthermore, the extracted polysaccharides
not only had anti-tumor activity against mouse S180 sarcoma cells as indicated by its inhibition on the S180 sarcoma cellderived
tumor growth by 26.62%–63.01%, but also significantly increased both thymus index and spleen index in mice.

Key words: response surface methodology, Crifola frondosa, fermentation broth, polysaccharide, microwave-assisted extraction, anti-tumor activity